Journal: Nutrients

Article Title: Impact of 2′-Fucosyllactose on Gut Microbiota Composition in Adults with Chronic Gastrointestinal Conditions: Batch Culture Fermentation Model and Pilot Clinical Trial Findings

doi: 10.3390/nu13030938

Figure Lengend Snippet: 16S rRNA-Targeted Oligonucleotide Probes Used for In Vitro Experiments.

Article Snippet: Stool samples were shipped to Genova Diagnostics (Asheville, NC, USA) within twenty-four hours of collection; commensal gut microbiota were identified using 16S ribosomal RNA gene PCR and concentrations of SCFA were measured using gas chromatography–mass spectrometry (GC-MS) as previously described [ ].

Techniques: In Vitro, Sequencing, Negative Control, Bacteria

Journal:

Article Title: Pneumolysin Detection Identifies Atypical Isolates of Streptococcus pneumoniae

doi:

Figure Lengend Snippet: Results of confirmatory tests on five isolates of S. pneumoniae with atypical colonial appearances

Article Snippet: We therefore examined further these five isolates (i) by a real-time PCR method for the pneumolysin gene which included a hybridization probe for amplicon recognition ( 4 ), (ii) by demonstrating the expression of pneumolysin in the lysed organisms using a monoclonal antibody to a recombinant pneumolysin in a Western blot technique ( 8 ), and (iii) by a commercial 16S RNA gene probe (AccuProbe; Gene-Probe Inc., San Diego, Calif.).

Techniques: Western Blot, Solubility